fxcycle violet dna stain (Thermo Fisher)
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Fxcycle Violet Dna Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fxcycle+violet+dna+stain/DNA/pm41422504-786-23-27
Average 99 stars, based on 1 article reviews
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Flow Cytometry:Article Title: Monitoring Spontaneous Quiescence and Asynchronous Proliferation-Quiescence Decisions in Prostate Cancer Cells Article Snippet: Appendix 3: Additional Detailed Methods: Western Blotting (Supplemental Fig. 2) Antibodies used: anti-mouse Rb2/p130 (BD Biosciences, 610261,1:1000), anti-phospho-RB (Cell Signaling, #9308), anti-mouse phospho-Cdk2T160 (Cell Signaling, 1:500), anti-mouse Cdk2(Santa Cruz, M2, 1:1000), anti-rabbit p27 (Cell Signaling, #2552, 1:1000) or anti-mouse GAPDH (Cell Signaling, 14C10, 1:2000) were used as loading controls with the appropriate .. For flow cytometry analysis of DNA content, cells were fixed with 4% paraformaldehyde, and then stained with the Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma Article Snippet: .. After the culture, cells were stained with 5-ethynyl-2'-deoxyuridine (EdU) and Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells. Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma. Article Snippet: Background Nasopharyngeal carcinoma (NPC) cells express high levels of epidermal growth factor receptor (EGFR).. Cetuximab is an anti-EGFR monoclonal antibody that promotes natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC) via engagement of CD16.. We studied safety and efficacy of combining cetuximab with autologous expanded NK cells in patients with recurrent and/or metastatic NPC who had failed at least two prior lines of chemotherapy. Staining:Article Title: Monitoring Spontaneous Quiescence and Asynchronous Proliferation-Quiescence Decisions in Prostate Cancer Cells Article Snippet: Appendix 3: Additional Detailed Methods: Western Blotting (Supplemental Fig. 2) Antibodies used: anti-mouse Rb2/p130 (BD Biosciences, 610261,1:1000), anti-phospho-RB (Cell Signaling, #9308), anti-mouse phospho-Cdk2T160 (Cell Signaling, 1:500), anti-mouse Cdk2(Santa Cruz, M2, 1:1000), anti-rabbit p27 (Cell Signaling, #2552, 1:1000) or anti-mouse GAPDH (Cell Signaling, 14C10, 1:2000) were used as loading controls with the appropriate .. For flow cytometry analysis of DNA content, cells were fixed with 4% paraformaldehyde, and then stained with the Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma Article Snippet: .. After the culture, cells were stained with 5-ethynyl-2'-deoxyuridine (EdU) and Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation Article Snippet: .. Fixed cells were incubated with either Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells. Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of Article Title: SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells Article Snippet: .. For cell-cycle analysis, cells were incubated with antibodies against extracellular markers, fixated/permeabilized (BD Cytofix/Cytoperm kit), and intracellularly stained with anti-KI67 and Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma. Article Snippet: Background Nasopharyngeal carcinoma (NPC) cells express high levels of epidermal growth factor receptor (EGFR).. Cetuximab is an anti-EGFR monoclonal antibody that promotes natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC) via engagement of CD16.. We studied safety and efficacy of combining cetuximab with autologous expanded NK cells in patients with recurrent and/or metastatic NPC who had failed at least two prior lines of chemotherapy. Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation. Article Snippet: .. Fixed cells were incubated with either Incubation:Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation Article Snippet: .. Fixed cells were incubated with either Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells. Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of Article Title: SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells Article Snippet: .. For cell-cycle analysis, cells were incubated with antibodies against extracellular markers, fixated/permeabilized (BD Cytofix/Cytoperm kit), and intracellularly stained with anti-KI67 and Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation. Article Snippet: .. Fixed cells were incubated with either FACS:Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells. Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of Cell Cycle Assay:Article Title: SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells Article Snippet: .. For cell-cycle analysis, cells were incubated with antibodies against extracellular markers, fixated/permeabilized (BD Cytofix/Cytoperm kit), and intracellularly stained with anti-KI67 and |

