fxcycle violet dna stain  (Thermo Fisher)

Journal: Molecular Systems Biology

Article Title: A ubiquitous GC content signature underlies multimodal mRNA regulation by DDX3X

doi: 10.1038/s44320-024-00013-0

Figure Lengend Snippet: Reagents and tools.

Article Snippet: FxCycle Violet DNA content stain , Thermo Fisher , Cat# F10347.

Techniques: Sequencing, Software, Flow Cytometry, Staining, Real-time Polymerase Chain Reaction

Journal: Cancer Research Communications

Article Title: The MDM2 Inhibitor Navtemadlin Arrests Mouse Melanoma Growth In Vivo and Potentiates Radiotherapy

doi: 10.1158/2767-9764.CRC-22-0053

Figure Lengend Snippet: Navtemadlin inhibits murine cell growth in a p53-dependent manner. A–E, Cell growth, measured as % confluence over time, of B16-F10 p53 +/+ and p53 −/− mouse melanoma cells, YUMM 1.7 mouse melanoma cells, CT26.WT mouse colon carcinoma cells, and NIH/3T3 mouse fibroblast cells treated with indicated concentrations of Navtemadlin or vehicle control was monitored for 96 hours (IncuCyte S3 system). F, Representative plots showing flow cytometry analyses of cell-cycle measuring incorporation of EdU into newly synthesized DNA after 72 hours treatment with 1.5 μmol/L Navtemadlin. DNA was stained with FxCycle. The gates were based on FMO controls. G and H, Cell-cycle phases of B16-F10 p53 +/+ and p53 −/− cells 24, 48, and 72 hours after treatment with 1.5 μmol/L Navtemadlin based on EdU incorporation in proliferating cells. The data are representative of at least two experiments performed with triplicates. Mean ± SD. *, P <0.05; ****, P < 0.0001. One-way ANOVA.

Article Snippet: After washing, single-cell suspensions were resuspended in flow buffer (0.5% BSA, 2 μmol/L EDTA in PBS) containing the DNA stain FxCycle Violet (1:2,000 dilution, Thermo Fisher Scientific).

Techniques: Control, Flow Cytometry, Synthesized, Staining