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fxcycle violet dna stain  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fxcycle violet dna stain
    Fxcycle Violet Dna Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fxcycle+violet+dna+stain/DNA/pm41422504-786-23-27
    Average 99 stars, based on 1 article reviews
    fxcycle violet dna stain - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Monitoring Spontaneous Quiescence and Asynchronous Proliferation-Quiescence Decisions in Prostate Cancer Cells
    Article Snippet: Appendix 3: Additional Detailed Methods: Western Blotting (Supplemental Fig. 2) Antibodies used: anti-mouse Rb2/p130 (BD Biosciences, 610261,1:1000), anti-phospho-RB (Cell Signaling, #9308), anti-mouse phospho-Cdk2T160 (Cell Signaling, 1:500), anti-mouse Cdk2(Santa Cruz, M2, 1:1000), anti-rabbit p27 (Cell Signaling, #2552, 1:1000) or anti-mouse GAPDH (Cell Signaling, 14C10, 1:2000) were used as loading controls with the appropriate .. For flow cytometry analysis of DNA content, cells were fixed with 4% paraformaldehyde, and then stained with the FxCycle Violet DNA Stain (Thermo Fisher, F10347). ..

    Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma
    Article Snippet: .. After the culture, cells were stained with 5-ethynyl-2'-deoxyuridine (EdU) and FxCycle Violet DNA stain (both from Thermo Fisher Scientific), and the presence of dividing cells was excluded by flow cytometry. ..

    Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells.
    Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of FxCycle Violet DNA stain (Thermo Fisher Scientific) at room temperature for 30 min. Novocyte Quanteon flow cytometer (Agilent) was used to measure DNA content and to identify NaPi2b-positive 8CLCs. .. Cell cycle analysis was performed using NovoExpress software (Agilent).

    Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma.
    Article Snippet: Background Nasopharyngeal carcinoma (NPC) cells express high levels of epidermal growth factor receptor (EGFR).. Cetuximab is an anti-EGFR monoclonal antibody that promotes natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC) via engagement of CD16.. We studied safety and efficacy of combining cetuximab with autologous expanded NK cells in patients with recurrent and/or metastatic NPC who had failed at least two prior lines of chemotherapy.

    Staining:

    Article Title: Monitoring Spontaneous Quiescence and Asynchronous Proliferation-Quiescence Decisions in Prostate Cancer Cells
    Article Snippet: Appendix 3: Additional Detailed Methods: Western Blotting (Supplemental Fig. 2) Antibodies used: anti-mouse Rb2/p130 (BD Biosciences, 610261,1:1000), anti-phospho-RB (Cell Signaling, #9308), anti-mouse phospho-Cdk2T160 (Cell Signaling, 1:500), anti-mouse Cdk2(Santa Cruz, M2, 1:1000), anti-rabbit p27 (Cell Signaling, #2552, 1:1000) or anti-mouse GAPDH (Cell Signaling, 14C10, 1:2000) were used as loading controls with the appropriate .. For flow cytometry analysis of DNA content, cells were fixed with 4% paraformaldehyde, and then stained with the FxCycle Violet DNA Stain (Thermo Fisher, F10347). ..

    Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma
    Article Snippet: .. After the culture, cells were stained with 5-ethynyl-2'-deoxyuridine (EdU) and FxCycle Violet DNA stain (both from Thermo Fisher Scientific), and the presence of dividing cells was excluded by flow cytometry. ..

    Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation
    Article Snippet: .. Fixed cells were incubated with either FxCycle Violet DNA stain (4,6-diamidino- 2-phenylindole dihydrochloride) (Thermo Fisher Scientific) in 0.1% BSA/PBS overnight at 4 °C, or 20 μg/ml propidium iodide (PI) in 1% Triton X-100/PBS and 0.1 mg/ml RNase A overnight at room temperature. ..

    Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells.
    Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of FxCycle Violet DNA stain (Thermo Fisher Scientific) at room temperature for 30 min. Novocyte Quanteon flow cytometer (Agilent) was used to measure DNA content and to identify NaPi2b-positive 8CLCs. .. Cell cycle analysis was performed using NovoExpress software (Agilent).

    Article Title: SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells
    Article Snippet: .. For cell-cycle analysis, cells were incubated with antibodies against extracellular markers, fixated/permeabilized (BD Cytofix/Cytoperm kit), and intracellularly stained with anti-KI67 and FxCycle Violet DNA stain (Thermo Fisher Scientific). ..

    Article Title: Phase I study of expanded natural killer cells in combination with cetuximab for recurrent/metastatic nasopharyngeal carcinoma.
    Article Snippet: Background Nasopharyngeal carcinoma (NPC) cells express high levels of epidermal growth factor receptor (EGFR).. Cetuximab is an anti-EGFR monoclonal antibody that promotes natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC) via engagement of CD16.. We studied safety and efficacy of combining cetuximab with autologous expanded NK cells in patients with recurrent and/or metastatic NPC who had failed at least two prior lines of chemotherapy.

    Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation.
    Article Snippet: .. Fixed cells were incubated with either FxCycle Violet DNA stain (4,6-diamidino- 2- phenylindole dihydrochloride) (Thermo Fisher Scientific) in 0.1% BSA/PBS overnight at 4oC, or 20 μg/ml propidium iodide (PI) in 1% Triton X-100/PBS and 0.1 mg/ml RNase A overnight at room temperature. ..

    Incubation:

    Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation
    Article Snippet: .. Fixed cells were incubated with either FxCycle Violet DNA stain (4,6-diamidino- 2-phenylindole dihydrochloride) (Thermo Fisher Scientific) in 0.1% BSA/PBS overnight at 4 °C, or 20 μg/ml propidium iodide (PI) in 1% Triton X-100/PBS and 0.1 mg/ml RNase A overnight at room temperature. ..

    Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells.
    Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of FxCycle Violet DNA stain (Thermo Fisher Scientific) at room temperature for 30 min. Novocyte Quanteon flow cytometer (Agilent) was used to measure DNA content and to identify NaPi2b-positive 8CLCs. .. Cell cycle analysis was performed using NovoExpress software (Agilent).

    Article Title: SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells
    Article Snippet: .. For cell-cycle analysis, cells were incubated with antibodies against extracellular markers, fixated/permeabilized (BD Cytofix/Cytoperm kit), and intracellularly stained with anti-KI67 and FxCycle Violet DNA stain (Thermo Fisher Scientific). ..

    Article Title: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation.
    Article Snippet: .. Fixed cells were incubated with either FxCycle Violet DNA stain (4,6-diamidino- 2- phenylindole dihydrochloride) (Thermo Fisher Scientific) in 0.1% BSA/PBS overnight at 4oC, or 20 μg/ml propidium iodide (PI) in 1% Triton X-100/PBS and 0.1 mg/ml RNase A overnight at room temperature. ..

    FACS:

    Article Title: Integrated transcriptomic analysis reveals metabolic remodeling and gene expression networks related to human 8-cell-stage embryo-like cells.
    Article Snippet: Cells were washed three times as above and then either FAC-sorted using Sony SH800Z Cell Sorter (Sony Biotechnology), with a 100 μm nozzle, or fixed with 4% paraformaldehyde (PFA) for 5 min on ice to prepare for cell cycle analysis. .. For cell cycle profiling, 1 × 10 6 NaPi2b-stained, PFA-fixed cells suspended in 500 μL FACS buffer were incubated with one drop of FxCycle Violet DNA stain (Thermo Fisher Scientific) at room temperature for 30 min. Novocyte Quanteon flow cytometer (Agilent) was used to measure DNA content and to identify NaPi2b-positive 8CLCs. .. Cell cycle analysis was performed using NovoExpress software (Agilent).

    Cell Cycle Assay:

    Article Title: SCA-1 Expression Level Identifies Quiescent Hematopoietic Stem and Progenitor Cells
    Article Snippet: .. For cell-cycle analysis, cells were incubated with antibodies against extracellular markers, fixated/permeabilized (BD Cytofix/Cytoperm kit), and intracellularly stained with anti-KI67 and FxCycle Violet DNA stain (Thermo Fisher Scientific). ..



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    Navtemadlin inhibits murine cell growth in a p53-dependent manner. A–E, Cell growth, measured as % confluence over time, of B16-F10 p53 +/+ and p53 −/− mouse melanoma cells, YUMM 1.7 mouse melanoma cells, CT26.WT mouse colon carcinoma cells, and NIH/3T3 mouse fibroblast cells treated with indicated concentrations of Navtemadlin or vehicle control was monitored for 96 hours (IncuCyte S3 system). F, Representative plots showing flow cytometry analyses of cell-cycle measuring incorporation of EdU into newly synthesized <t>DNA</t> after 72 hours treatment with 1.5 μmol/L Navtemadlin. DNA was stained with <t>FxCycle.</t> The gates were based on FMO controls. G and H, Cell-cycle phases of B16-F10 p53 +/+ and p53 −/− cells 24, 48, and 72 hours after treatment with 1.5 μmol/L Navtemadlin based on EdU incorporation in proliferating cells. The data are representative of at least two experiments performed with triplicates. Mean ± SD. *, P <0.05; ****, P < 0.0001. One-way ANOVA.
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    Image Search Results


    Reagents and tools.

    Journal: Molecular Systems Biology

    Article Title: A ubiquitous GC content signature underlies multimodal mRNA regulation by DDX3X

    doi: 10.1038/s44320-024-00013-0

    Figure Lengend Snippet: Reagents and tools.

    Article Snippet: FxCycle Violet DNA content stain , Thermo Fisher , Cat# F10347.

    Techniques: Sequencing, Software, Flow Cytometry, Staining, Real-time Polymerase Chain Reaction

    Navtemadlin inhibits murine cell growth in a p53-dependent manner. A–E, Cell growth, measured as % confluence over time, of B16-F10 p53 +/+ and p53 −/− mouse melanoma cells, YUMM 1.7 mouse melanoma cells, CT26.WT mouse colon carcinoma cells, and NIH/3T3 mouse fibroblast cells treated with indicated concentrations of Navtemadlin or vehicle control was monitored for 96 hours (IncuCyte S3 system). F, Representative plots showing flow cytometry analyses of cell-cycle measuring incorporation of EdU into newly synthesized DNA after 72 hours treatment with 1.5 μmol/L Navtemadlin. DNA was stained with FxCycle. The gates were based on FMO controls. G and H, Cell-cycle phases of B16-F10 p53 +/+ and p53 −/− cells 24, 48, and 72 hours after treatment with 1.5 μmol/L Navtemadlin based on EdU incorporation in proliferating cells. The data are representative of at least two experiments performed with triplicates. Mean ± SD. *, P <0.05; ****, P < 0.0001. One-way ANOVA.

    Journal: Cancer Research Communications

    Article Title: The MDM2 Inhibitor Navtemadlin Arrests Mouse Melanoma Growth In Vivo and Potentiates Radiotherapy

    doi: 10.1158/2767-9764.CRC-22-0053

    Figure Lengend Snippet: Navtemadlin inhibits murine cell growth in a p53-dependent manner. A–E, Cell growth, measured as % confluence over time, of B16-F10 p53 +/+ and p53 −/− mouse melanoma cells, YUMM 1.7 mouse melanoma cells, CT26.WT mouse colon carcinoma cells, and NIH/3T3 mouse fibroblast cells treated with indicated concentrations of Navtemadlin or vehicle control was monitored for 96 hours (IncuCyte S3 system). F, Representative plots showing flow cytometry analyses of cell-cycle measuring incorporation of EdU into newly synthesized DNA after 72 hours treatment with 1.5 μmol/L Navtemadlin. DNA was stained with FxCycle. The gates were based on FMO controls. G and H, Cell-cycle phases of B16-F10 p53 +/+ and p53 −/− cells 24, 48, and 72 hours after treatment with 1.5 μmol/L Navtemadlin based on EdU incorporation in proliferating cells. The data are representative of at least two experiments performed with triplicates. Mean ± SD. *, P <0.05; ****, P < 0.0001. One-way ANOVA.

    Article Snippet: After washing, single-cell suspensions were resuspended in flow buffer (0.5% BSA, 2 μmol/L EDTA in PBS) containing the DNA stain FxCycle Violet (1:2,000 dilution, Thermo Fisher Scientific).

    Techniques: Control, Flow Cytometry, Synthesized, Staining